Publications
Zhang J, Takacs C, McCausland J, Mueller E, Buron J, Thappeta Y, Wachter J, Rosa P, Jacobs-Wagner C (2024) Borrelia burgdorferi loses essential genetic elements and cell proliferative potential during stationary phase in culture but not in the tick vector. bioRxiv, October 2024 doi: https://doi.org/10.1101/2024.10.28.620338
Papagiannakis A, Yu Q, Govers S, Lin W, Wingreen N, Jacobs-Wagner C (2024) DNA/polysome phase separation and cell width confinement couple nucleoid segregation to cell growth in Escherichia coli. bioRxiv. October 2024 doi: https://doi.org/10.1101/2024.10.08.617237
Mäkelä J, Papagiannakis A, Lin W, C. Lanz M, Glenn S, Matthew S, K. MarinovJan G, Skotheim J, Jacobs-Wagner C (2024) Genome concentration limits cell growth and modulates proteome composition in Escherichia coli. Reviewed preprint. eLife.97465.1
Thappeta Y, J. Cañas-Duarte S, Kallem T, Fragasso A, Xiang Y, Gray W, Lee C, Cegelski L, Jacobs-Wagner C (2024) Glycogen phase separation drives macromolecular rearrangement and asymmetric division in E. coli. bioRxiv [Preprint]. 2024 Apr 20:2024.04.19.590186. doi: 10.1101/2024.04.19.590186.
Putnik R, Zhou J, Irnov I, Garner E, Liu M, Bersch K, Christine Jacobs-Wagner C and Leimkuhler Grimes C (2024) Synthesis of a Borrelia burgdorferi-Derived Muropeptide Standard Fragment Library. Molecules 2024, 29, 3297.
Jacobs-Wagner C (2024) Through the looking glass: An adventure into the metastable world of the bacterial cytoplasm. Cell, 18, 228-234.
Govers SK, Campos M, Tyagi B, Laloux G, Jacobs-Wagner C (2024) Apparent simplicity and emergent robustness in bacterial cell cycle control. Cell Systems, 15, 19-36.
Carlson D, Kowalewski M, Bodoor K, Lietzan A, Hughes P, Gooden D, Loiselle D, Alcorta D, Dingman Z, Mueller Z, Irnov I, Modla S, Chaya T, Caplan J, Embers M, Miller J, Jacobs-Wagner C, Redinbo M, Spector N, Haystead T (2023) Targeting Borrelia burgdorferi HtpG with a berserker molecule, a strategy for anti-microbial development. Cell Chem Biol . 2023 Nov 1:S2451-9456(23)00340-9. doi: 10.1016/j.chembiol.2023.10.004.
Ren Z, Takacs CN, Brandão HB, Jacobs-Wagner C, Wang X (2023) Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferi. PLoS Genet 19(7): e1010857.
Takacs CN, Nakajima Y, Haber JE, Jacobs-Wagner C (2022) Cas9-mediated endogenous plasmid loss in Borrelia burgdorferi. PLoS ONE 17(11): e0278151.
Takacs CN, Wachter J, Xiang Y, Ren Z, Karaboja X, Scott M, Stoner MR, Irnov I, Jannetty N, Rosa PA, Wang X, Jacobs-Wagner C (2022) Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete. Nat Commun 13:7173.
pdf Correction Editor's Highlight
Lin WH, Jacobs-Wagner C (2022) Connecting single-cell ATP dynamics to overflow metabolism, cell growth and the cell cycle in Escherichia coli. Curr Biol 32(18):3911-3924
Jacobs-Wagner C (2021) Christine Jacobs-Wagner Q&A. Curr Biol 31, 14: R882-883.
Xiang Y, Surovtsev IV, Chang Y, Govers SK, Parry BR, Liu J, Jacobs-Wagner C (2021) Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli. Cell 184: 3626-3642.
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C (2021) A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Appl Envir Microbiol, 87(4): e02519-20.
Govers SK, Jacobs-Wagner C (2020) Caulobacter crescentus: model system extraordinaire. Curr Biol 30, 19: R1151-R1158.
Gupta A, Arora G, Rosen CE, Kloos Z, Cao Y, Cerny J, Sajid A, Hoornstra D, Golovchenko M, Rudenko N, Munderloh U, Hovius JW, Booth CJ, Jacobs-Wagner C, Palm NW, Ring AM, Fikrig E (2020) A human secretome library screen reveals a role for Peptidoglycan 1 Recognition Protein in Lyme borreliosis. PLOS Pathogen 16(11): e1009030.
Lin WH, Kussell E, Young LS, Jacobs-Wagner C (2020) Origin of exponential growth in nonlinear reaction networks. Proc Natl Acad Sci USA 20201306.
Sanchez AD, Branon TC, Cote LE, Papagiannakis A, Liang X, Shen K, Jacobs-Wagner C, Ting AY, Feldman JL (2020) Proximity labeling reveals non-centrosomal microtubule-organizing center components required for microtubule growth and localization. Curr Biol 31: 1-15.
Kim S, Beltran B, Irnov I, Jacobs-Wagner C (2019) Long-distance cooperative and antagonistic RNA polymerase dynamics via DNA supercoiling. Cell 179:106-119.
PubMed pdf Supplemental code F1000 Commentary
Jutras BL, Lochhead RB, Kloos ZA, Biboy J, Strle K, Booth CJ, Govers SK, Gray J, Schumann P, Vollmer W, Bockenstedt LK, Steere AC, Jacobs-Wagner C (2019) Borrelia burgdorferi peptidoglycan is a persistent antigen in patients with Lyme arthritis. Proc Natl Acad Sci USA pii: 201904170.
Gray WT, Govers SK, Xiang Y, Parry BR, Campos M, Kim S, Jacobs-Wagner C (2019) Nucleoid size scaling and intracellular organization of translation across bacteria. Cell 177(6):1632-1648.
Pubmed pdf Supplemental code News Commentary F1000
Shen H, Fallas JA, Lynch E, Sheffler W, Parry BR, Jannetty N, Decarreau J, Wagenbach M, Vicente JJ, Chen J, Wang L, Dowling Q, Oberdorfer G, Stewart L, Wordeman L, De Yoreo J, Jacobs-Wagner C, Kollman J, Baker D (2018) De Novo design of self-assembling helical protein filaments. Science 362(6415):705-709.
Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C (2018) Characterization of fluorescent proteins, promoters, and selectable markers for applications in the Lyme disease spirochete Borrelia burgdorferi. Appl Environ Microbiol. 84(24):e01824-18
Campos M, Govers SK, Irnov I, Dobihal GS, Cornet F, Jacobs-Wagner C (2018) Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli. Mol Syst Biol 25;14(6).
PubMed pdf Supplemental data Supplemental code
Delarue M, Brittingham GP, Pfeffer S, Surovtsev IV, Pinglay S, Kennedy KJ, Schaffer M, Gutierrez JI, Sang D, Poterewicz G, Chung JK, Plitzko JM, Groves JT, Jacobs-Wagner C, Engel BD, Holt LJ (2018) mTORC1 controls phase separation and the biophysical properties of the cytoplasm by tuning crowding. Cell 174(2):338-349.
Kim S, Jacobs-Wagner C (2018) Effects of mRNA degradation and site-specific transcriptional pausing on protein expression noise. Biophys J 114(7):1718-1729.
Surovtsev IV, Jacobs-Wagner C (2018) Subcellular organization: A critical feature of bacterial cell replication. Cell 172(6):1271-1293.
Irnov I, Wang Z, Jannetty ND, Bustamante JA, Rhee KY, Jacobs-Wagner C (2017) Crosstalk between the tricarboxylic acid cycle and peptidoglycan synthesis in Caulobacter crescentus through the homeostatic control of α-ketoglutarate. PLoS Genet 13(8):e1006978.
Abraham NM, Liu L, Jutras BL, Murfin K, Acar A, Yarovinsky TO, Sutton E, Heisig M, Jacobs-Wagner C, Fikrig E (2017) A tick antivirulence protein potentiates antibiotics against Staphylococcus aureus. Antimicrob Agents Chemother 61(7):e00113-17.
Abraham NM, Liu L, Jutras BL, Yadav AK, Narasimhan S, Gopalakrishnan V, Ansari JM, Jefferson KK, Cava F, Jacobs-Wagner C, Fikrig E (2017) Pathogen-mediated manipulation of arthropod microbiota to promote infection. Proc Natl Acad Sci USA 114(5):E781-E790.
Arias-Cartin R, Dobihal GS, Campos M, Surovtsev IV, Parry B, Jacobs-Wagner C (2017) Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter. EMBO J 36(3):301-318.
Surovtsev IV, Campos M, Jacobs-Wagner C (2016) DNA-relay mechanism is sufficient to explain ParA-dependent intracellular transport and patterning of single and multiple cargos. Proc Natl Acad Sci USA 113(46):E7268-E7276.
Jutras BL, Scott M, Parry B, Biboy J, Gray J, Vollmer W, Jacobs-Wagner C (2016) Lyme disease and relapsing fever Borrelia elongate through zones of peptidoglycan synthesis that mark division sites of daughter cells. Proc Natl Acad Sci USA 113(33):9162-70.
Surovtsev IV, Lim HC, Jacobs-Wagner C (2016) The slow mobility of the ParA partitioning protein underlies its steady-state patterning in Caulobacter. Biophys J 110(12):2790-9.
Paintdakhi A, Parry B, Campos M, Irnov I, Elf J, Surovtsev I, Jacobs-Wagner C (2016) Oufti: An integrated software package for high-accuracy, high-throughput quantitative microscopy analysis. Mol Microbiol 99(4):767-77.
Huang F, Sirinakis G, Allgeyer ES, Schroeder LK, Duim WC, Kromann EB, Phan T, Rivera-Molina FE, Myers JR, Irnov I, Lessard M, Zhang Y, Handel MA, Jacobs-Wagner C, Lusk CP, Rothman JE, Toomre D, Booth MJ, Bewersdorf J. (2016) Ultra-High Resolution 3D Imaging of Whole Cells. Cell 166(4):1028-49.
Jutras BL, Jacobs-Wagner C (2015) Bacterial evolution: What goes around comes around. Curr Biol 25:R496-8.
Chou S, Daugherty MD, Peterson SB, Biboy J, Yang Y, Jutras BL, Fritz-Laylin LK, Ferrin MA, Harding BN, Jacobs-Wagner C, Yang XF, Vollmer W, Malik HS, Mougous JD (2015) Transferred interbacterial antagonism genes augment eukaryotic innate immune function. Nature 518: 99-101.
Alpha CJ, Campos M, Jacobs-Wagner C, Strobel SA (2015) Mycofumigation by the volatile organic compound producing fungus Muscodor albus induces bacterial cell death through DNA damage. Appl Environ Microbiol 81:1147-56.
Campos M, Surovtsev IV, Kato S, Paintdakhi A, Beltran B, Ebmeier SE, Jacobs-Wagner C (2014) A constant size extension drives bacterial cell size homeostasis. Cell 159:1433-46.
PubMed pdf Supplemental code Supplemental data
Lim HC, Surovtsev IV, Beltran BG, Huang F, Bewersdorf J, Jacobs-Wagner C (2014) Evidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation. Elife 3:e02758.
Deghelt M, Mullier C, Sternon JF, Francis N, Laloux G, Dotreppe D, Van der Henst C, Jacobs-Wagner C, Letesson JJ, De Bolle X (2014) G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus. Nat Commun 5:4366.
Parry BR, Surovtsev IV, Cabeen MT, O'Hern CS, Dufresne ER, Jacobs-Wagner C (2014) The bacterial cytoplasm has glass-like properties and is fluidized by metabolic activity. Cell 156:183-94.
Laloux G, Jacobs-Wagner C (2014) How do bacteria localize proteins to the cell pole? J Cell Sci 127:11-9.
Ko JH, Montero Llopis PM, Heinritz J, Jacobs-Waner C, Soll D (2013) Suppression of amber codons in Caulobacter crescentus by the orthogonal Escherichia coli Histidyl-tRNA synthetase/tRNAHis pair. PloS One 8:e83630.
Fang G, Passalacqua K, Hocking J, Montero Llopis P, Gerstein M, Bergman NH, and Jacobs-Wagner C (2013) Transcriptomic and phylogenetic analysis of a bacterial cell cycle reveals strong associations between gene co-expression and evolution. BMC Genomics 14:450.
Laloux G and Jacobs-Wagner C. (2013) Spatiotemporal control of PopZ localization through cell cycle-coupled multimerization. J Cell Biol 201:827-41.
Takacs CN, Hocking J, Cabeen MT, Bui NK, Poggio S, Vollmer W, and Jacobs-Wagner C. (2013) Growth medium-dependent glycine incorporation into the peptidoglycan of Caulobacter crescentus. PloS One 8(2):e57579.
Campos M and Jacobs-Wagner C. (2013) Cellular organization of the transfer of genetic information. Curr Opin Microbiol 16:171-6.
Montero Llopis P, Sliusarenko O, Heinritz J, and Jacobs-Wagner C. (2012) In vivo biochemistry in bacterial cells using FRAP: insight into the translation cycle. Biophys J 103: 1848-59.
Anwari K, Webb CT, Poggi S, Perry AJ, Bousoff M, Celik N, Ramm G, Lovering A, Sockett RE, Smit J, Jacobs-Wagner C, Lithgow T. (2012) The evolution of new lipoprotein subunits of the bacterial outer membrane BAM complex. Mol Microbiol 84:832-44.
Hocking J, Priyadarshini R, Takacs NC,Costa T, Dye N, Vollmer W, and Jacobs-Wagner C. (2012) Osmolarity-dependent relocation of penicillin-binding protein PBP2 to the division machinery in Caulobacter crescentus. J Bacteriol 194:3116-27.
Charbon G, Wang J, Brustad E, Schultz PG, Horwich , Jacobs-Wagner C, Chapman E. (2011) Localization of GroEL determined by in vivo incorporation of fluorescent amino acid. Bioorg Med Chem Lett 211:6067-70.
Charbon G, Brustad E, Scott KA, Wang J, Løbner-Olesen A, Schultz PG, Jacobs-Wagner C, Chapman E. (2011) Subcellular protein localization by using a genetically encoded fluorescent amino Acid. Chembiochem 12:1818-21.
Sliusarenko O, Heinritz J, Emonet T, Jacobs-Wagner C. (2011) High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics. Mol Microbiol; 80:612-27.
Cabeen MT, Herrmann H, Jacobs-Wagner C. (2011) The domain organization of the bacterial intermediate filament-like protein crescentin is important for assembly and function. Cytoskeleton (Hoboken); 68:205-19.
Cabeen MT, Jacobs-Wagner C. (2010) The bacterial cytoskeleton. Annu Rev Genet; 44:365-92.
Schofield WB, Lim HC, Jacobs-Wagner C. (2010) Cell cycle coordination and regulation of bacterial chromosome segregation dynamics by polarly localized proteins. EMBO J; 29:3068-81.
Cabeen MT, Jacobs-Wagner C. (2010) A metabolic assembly line in bacteria. Nat Cell Biol; 12:731-3.
Montero Llopis PM, Jackson AF, Sliusarenko O, Surovtsev I, Heinritz J, Emonet T, Jacobs-Wagner C. (2010) Spatial organization of the flow of genetic information in bacteria. Nature; 466: 77-81.
Poggio S, Takacs CN, Vollmer W, Jacobs-Wagner C. (2010) A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. Mol Microbiol; 77:74-89
Cabeen MT, Murolo MA, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C. (2010) Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus. J Bacteriol;192: 3368-78.
Sliusarenko O, Cabeen MT, Wolgemuth CW, Jacobs-Wagner C, Emonet T. (2010) Processivity of peptidoglycan synthesis provides a built-in mechanism for the robustness of straight-rod cell morphology. Proc Natl Acad Sci U S A;107: 10086-91.
Anwari K, Poggio S, Perry A, Gatsos X, Ramarathinam SH, Williamson NA, Noinaj N, Buchanan S, Gabriel K, Purcell AW, Jacobs-Wagner C, Lithgow T. (2010) A modular BAM complex in the outer membrane of the alpha-proteobacterium Caulobacter crescentus. PLoS One 5, e8619.
Takacs CN, Poggio S, Charbon G, Pucheault M, Vollmer W, Jacobs-Wagner C. (2010) MreB drives de novo rod morphogenesis in Caulobacter crescentus via remodeling of the cell wall.
J Bacteriol. 192, 1671-84.
Angelastro PS, Sliusarenko O, Jacobs-Wagner C. (2010) Polar localization of the CckA histidine kinase and cell cycle periodicity of the essential master regulator CtrA in Caulobacter crescentus. J Bacteriol. 192, 539-52.
Clements A, Bursac D, Gatsos X, Perry AJ, Civciristov S, Celik N, Likic VA, Poggio S, Jacobs-Wagner C, Strugnell RA, Lithgow T. (2009) The reducible complexity of a mitochondrial molecular machine. Proc Natl Acad Sci U S A. 106, 15791-5.
Cabeen MT, Charbon G, Vollmer W, Born P, Ausmees A, Weibel, D and Jacobs-Wagner C. (2009) Bacterial cell curvature via mechanical control of cell growth. EMBO J 28, 1208-1219.
Charbon G, Cabeen MT and Jacobs-Wagner C. (2009) Bacterial intermediate filaments: In vivo assembly, organization and dynamics of crescentin. Genes & Dev 23, 1131-44.
Alyahya S.A, Alexander A, Costa T, Henriques AO, Emonet T, Jacobs-Wagner C. (2009) RodZ, a component of the bacterial core morphogenic apparatus. Proc Natl Acad Sci USA 106:1239-44.
Costa T, Priyadarshini R, Jacobs-Wagner C. (2008) Localization of PBP3 in Caulobacter crescentus is highly dynamic and largely relies on its functional transpeptidase domain. Mol Microbiol. 70:634-651.
Ebersbach G, Briegel A, Jensen GJ, Jacobs-Wagner C. (2008) A self-associating protein critical for chromosome attachment, division, and polar organization in Caulobacter. Cell 134:956-68.
PubMed pdf Supplemental Cell Preview
Obuchowski PL, Jacobs-Wagner C. (2008) PflI, a protein involved in flagellar positioning in Caulobacter crescentus.
J Bacteriol. 190:1718-29.
Cabeen MT, Jacobs-Wagner C. (2007) Skin and bones: the bacterial cytoskeleton, cell wall, and cell morphogenesis.
J Cell Biol. 179:381-7.
Aaron M, Charbon G, Lam H, Schwarz H, Vollmer W, Jacobs-Wagner C. (2007) The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus. Mol Microbiol. 64:938-52.
PubMed pdf Supplemental Molecular Microbiology Commentary
Hallez R, Mignolet J, Van Mullem V, Wery M, Vandenhaute J, Letesson JJ, Jacobs-Wagner C, De Bolle X. (2007) The asymmetric distribution of the essential histidine kinase PdhS indicates a differentiation event in Brucella abortus. EMBO J. 26:1444-55.
Ebersbach G, Jacobs-Wagner C. (2007) Exploration into the spatial and temporal mechanisms of bacterial polarity. Trends Microbiol. 15:101-8.
Lam H., Schofield W.B. and Jacobs-Wagner, C. (2006) A landmark protein essential for establishing and perpetuating the polarity of a bacterial cell. Cell124:1011-23.
PubMed pdf Supplemental Cell Preview
Cabeen, M.T. and Jacobs-Wagner, C. (2005) Bacterial cell shape. Nat Rev Microbiol. 3:601-10
Matroule J.Y., Lam H., Burnette D.T., and Jacobs-Wagner C. (2004) Cytokinesis monitoring during development: Rapid pole-to-pole shuttling of a signaling protein by localized kinase and phosphatase in Caulobacter. Cell 118: 579-90.
PubMed pdf Nature Micro Review
Jacobs-Wagner, C. (2004) Regulatory proteins with a sense of direction: Cell cycle signaling network in Caulobacter. Mol Microbiol. 51:7-13.
Ausmees, N., Kuhn, J. R., and Jacobs-Wagner, C. (2003) The Bacterial cytoskeleton: An Intermediate Filament-like Function in Cell Shape. Cell 115:705-13.
PubMed pdf Science News Nature Micro Review Cell Preview Current Biology Dispatch
Ausmees, N. and Jacobs-Wagner, C. (2003) Spatial and temporal control of differentiation and cell cycle progression in Caulobacter crescentus. Annu Rev Microbiol. 57, 225-247.
Lam, H., Matroule, J.-Y., and Jacobs-Wagner, C. (2003) The asymmetric spatial distribution of bacterial signal transduction proteins coordinates cell cycle events. Dev Cell 5, 149-159.
Jacobs C, Ausmees N, Cordwell SJ, Shapiro L, Laub MT. (2003) Functions of the CckA histidine kinase in Caulobacter cell cycle control. Mol Microbiol.47:1279-90.